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polyclonal goat anti basic fibroblast growth factor fgf 2 antibody  (R&D Systems)


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    R&D Systems polyclonal goat anti basic fibroblast growth factor fgf 2 antibody
    Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor <t>(FGF-2)</t> (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.
    Polyclonal Goat Anti Basic Fibroblast Growth Factor Fgf 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti+basic+fibroblast+growth+factor+fgf+2+antibody/FITC+Antibody/pm17680989-50-64-73
    Average 95 stars, based on 494 article reviews
    polyclonal goat anti basic fibroblast growth factor fgf 2 antibody - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "Activation of phospholipase C pathways by a synthetic chondroitin sulfate-E tetrasaccharide promotes neurite outgrowth of dopaminergic neurons."

    Article Title: Activation of phospholipase C pathways by a synthetic chondroitin sulfate-E tetrasaccharide promotes neurite outgrowth of dopaminergic neurons.

    Journal: Journal of neurochemistry

    doi: 10.1111/j.1471-4159.2007.04849.x

    Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor (FGF-2) (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.
    Figure Legend Snippet: Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor (FGF-2) (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.

    Techniques Used: Derivative Assay, Cell Culture, Immunostaining, Confocal Laser Scanning Microscopy

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    Modification:

    Article Title: Activation of phospholipase C pathways by a synthetic chondroitin sulfate-E tetrasaccharide promotes neurite outgrowth of dopaminergic neurons.
    Article Snippet: .. Other reagents were obtained from the following sources: polyclonal rabbit anti-tyrosine hydroxylase (TH) antibody from Pel-Freeze (Rogers, AK, USA); polyclonal sheep anti-TH antibody from Chemicon (Temecula, CA, USA); polyclonal goat anti-midkine antibody, polyclonal goat anti-PTN antibody, polyclonal rabbit anti-BDNF antibody, polyclonal rabbit anti-protein tyrosine phosphatase f (PTPf) antibody and polyclonal goat antityrosine kinase B receptor (TrkB) antibody from Santa Cruz (Santa Cruz, CA, USA); polyclonal goat anti-basic fibroblast growth factor (FGF-2) antibody from R&D Systems (Minneapolis, MN, USA); FITC-conjugated anti-rabbit IgG, FITC-conjugated anti-sheep IgG, rhodamine red-conjugated anti-goat IgG and rhodamine red-conjugated anti-mouse IgG from Jackson ImmunoResearch (Baltimore, PA, USA); Dulbecco’s modified eagle medium, N2 supplement and fetal bovine serum from Invitrogen (Grand Island, NY, USA); minimal eagle’s medium, poly-L-ornithine (PLO), deoxyribonuclease I, soy bean trypsin inhibitor type II, bovine serum albumin, bisindolylmaleimide II, xestospongin C, BAPTA-AM and H89 from Sigma (St. Louis, MO, USA); U73122 from Tocris Cookson (Bristol, UK); cyclosporin A from Alexis Biochemicals (San Diego, CA, USA); KN62 from Seikagaku. ..



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    polyclonal goat anti basic fibroblast growth factor fgf 2 antibody  (R&D Systems)
    95
    R&D Systems polyclonal goat anti basic fibroblast growth factor fgf 2 antibody
    Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor <t>(FGF-2)</t> (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.
    Polyclonal Goat Anti Basic Fibroblast Growth Factor Fgf 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti+basic+fibroblast+growth+factor+fgf+2+antibody/FITC+Antibody/pm17680989-50-64-73
    Average 95 stars, based on 1 article reviews
    polyclonal goat anti basic fibroblast growth factor fgf 2 antibody - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

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    Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor (FGF-2) (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.

    Journal: Journal of neurochemistry

    Article Title: Activation of phospholipase C pathways by a synthetic chondroitin sulfate-E tetrasaccharide promotes neurite outgrowth of dopaminergic neurons.

    doi: 10.1111/j.1471-4159.2007.04849.x

    Figure Lengend Snippet: Fig. 4 Effect of antibodies for midkine- pleiotrophin (PTN)/protein tyrosine phosphatase f (PTPf) and brain-derived neurotrophic factor (BDNF)/tyrosine kinase B receptor (TrkB) signaling on chondroitin sul- fate (CS)-E tetrasaccharide-induced neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons. (a) Mesencephalic cells were cul- tured for 2 days on glass coverslips coated with poly-L-ornithine (PLO) alone or CS-E tetrasaccharide (16 lg/mL). The culture medium was switched to that containing either antibody specific for midkine (4 lg/ mL), PTN (2 lg/mL), BDNF (1 lg/mL), basic fibroblast growth factor (FGF-2) (4 lg/mL), PTPf (2 lg/mL) orTrkB (1 lg/mL). These cells were cultured for 3 days in the presence of either antibody. The neurite out- growth of TH-positive neurons was analyzed as described in Fig. 1. Data represent mean ± SEM of 114–140 cells obtained from two inde- pendent experiments. ***p < 0.001 compared with PLO alone; p < 0.001 compared with CS-E tetrasaccharide. (b) Photomicro- graphs of mesencephalic cells after immunostaining with anti-TH, -PTPf and -TrkB antibodies. Fluorescent images were collected at 63X mag- nification by confocal laser scanning microscopy. Scale bar, 20 lm.

    Article Snippet: Other reagents were obtained from the following sources: polyclonal rabbit anti-tyrosine hydroxylase (TH) antibody from Pel-Freeze (Rogers, AK, USA); polyclonal sheep anti-TH antibody from Chemicon (Temecula, CA, USA); polyclonal goat anti-midkine antibody, polyclonal goat anti-PTN antibody, polyclonal rabbit anti-BDNF antibody, polyclonal rabbit anti-protein tyrosine phosphatase f (PTPf) antibody and polyclonal goat antityrosine kinase B receptor (TrkB) antibody from Santa Cruz (Santa Cruz, CA, USA); polyclonal goat anti-basic fibroblast growth factor (FGF-2) antibody from R&D Systems (Minneapolis, MN, USA); FITC-conjugated anti-rabbit IgG, FITC-conjugated anti-sheep IgG, rhodamine red-conjugated anti-goat IgG and rhodamine red-conjugated anti-mouse IgG from Jackson ImmunoResearch (Baltimore, PA, USA); Dulbecco’s modified eagle medium, N2 supplement and fetal bovine serum from Invitrogen (Grand Island, NY, USA); minimal eagle’s medium, poly-L-ornithine (PLO), deoxyribonuclease I, soy bean trypsin inhibitor type II, bovine serum albumin, bisindolylmaleimide II, xestospongin C, BAPTA-AM and H89 from Sigma (St. Louis, MO, USA); U73122 from Tocris Cookson (Bristol, UK); cyclosporin A from Alexis Biochemicals (San Diego, CA, USA); KN62 from Seikagaku.

    Techniques: Derivative Assay, Cell Culture, Immunostaining, Confocal Laser Scanning Microscopy